Fabrication of hollow porous PLGA microspheres using sucrose for controlled dual delivery of dexamethasone and BMP2

Young-Bock Shim; Hong-Hee Jung; Ju Woong Jang; Hee Seok Yang; Hojae Bae; Jong-Chul Park; Bogyu Choi; Soo-Hong Lee

Journal of Industrial and Engineering Chemistry, Vol.37, 101-106, May, 2016

DOI10.1016/j.jiec.2016.03.014 Export Citation

Fabrication of hollow porous PLGA microspheres using sucrose for controlled dual delivery of dexamethasone and BMP2

Young-Bock Shim^{1, 2}, Hong-Hee Jung^{2, 3}, Ju Woong Jang^{2, 4}, Hee Seok Yang⁵, Hojae Bae⁶, Jong-Chul Park³, Bogyu Choi^{1, 4, †}, and Soo-Hong Lee^{1, 4, †}

¹Department of Biomedical Science, CHA University, Bundang-gu, Gyeonggi-do 463-400, Republic of Korea
²The Institute of Biomaterial and Medical Engineering, Cellumed Co., Ltd, Geumchen-gu, Seoul 153-782, Republic of Korea
³Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea
⁴, Korea
⁵Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea
⁶Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea

E-mail:,

Hollow porous poly(D,L-lactide-co-glycolide) microspheres (HP-PLGA-MS) were manufactured using the water-in-oil-in-water double emulsion solvent evaporation method with sucrose as a novel porogen. Dexamethasone (DEX) was dissolved in the oil phase and loaded during the preparation of the HP-PLGA-MS. Bone morphogenetic protein-2 (BMP2) with type I collagen (Col1) was incorporated into HP-PLGA-MS using a simple immersion method. The release rate of BMP2 could be controlled by the amount of Col1. DEX and BMP2 showed a sustained release profile. These results showed that the HP-PLGA-MS could be fabricated successfully using sucrose and showed great potential as a controlled dual drug delivery system.

Keywords:PLGA;Hollow porous microspheres;Sucrose;Dexamethasone;BMP2

[References]

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[Referenced By]

[1] Langer R, Acc. Chem. Res., 33, 94 (2000)

[2] Gombotz WR, Pettit DK, Bioconjugate Chem., 6, 332 (1995)

[3] Goosen MF, O’Shea GM, Gharapetian HM, Chou S, Sun AM, Biotechnol. Bioeng., 27, 146 (1985)

[4] Park TG, Lee HY, Nam YS, J. Control. Release, 55, 181 (1998)

[5] Cohen S, Yoshioka T, Lucarelli M, Hwang LH, Langer R, Pharm. Res., 8, 713 (1991)

[6] Jain RA, Biomaterials, 21, 2475 (2000)

[7] Singh M, Shirley B, Bajwa K, Samara E, Hora M, O’Hagan D, J. Control. Release, 70, 21 (2001)

[8] Jones AJ, Putney S, Johnson OL, Cleland JL, Adv. Drug Deliv. Rev., 28, 71 (1997)

[9] McGinity JW, O’Donnell PB, Adv. Drug Deliv. Rev., 28, 25 (1997)

[10] Danhier F, Ansorena E, Silva JM, Coco R, Breton AL, Preat V, J. Control. Release, 161, 505 (2012)

[11] Anderson JM, Shive MS, Adv. Drug Deliv. Rev., 28, 5 (1997)

[12] Kim S, Kwag DS, Lee DJ, Lee ES, Macromol. Res., 24(2), 176 (2016)

[13] Cai Y, Chen Y, Hong X, Liu Z, Yuan W, Int. J. Nanomed., 8, 1111 (2013)

[14] Freiberg S, Zhu XX, Int. J. Pharm., 282, 1 (2004)

[15] Lin K, Chen L, Liu P, Zou Z, Zhang M, Shen Y, Qiao Y, Liu X, Chang J, Cryst. Eng. Comm., 15, 2999 (2013)

[16] Zhu Y, Shi J, Shen W, Dong X, Feng J, Ruan M, Li Y, Angew. Chem.-Int. Edit., 44, 5083 (2005)

[17] Liang Z, Ni R, Zhou J, Mao S, Drug Discov. Today, 20, 380 (2015)

[18] Harris LD, Kim BS, Mooney DJ, J. Biomed. Mater. Res., 42, 396 (1998)

[19] Shastri VP, Hildgen P, Langer R, Biomaterials, 24, 3133 (2003)

[20] Lee J, Oh YJ, Lee SK, Lee KY, J. Control. Release, 146, 61 (2010)

[21] Cheng D, Cao X, Gao H, Wang Y, J. Mater. Chem. B, 1, 3322 (2013)

[22] Canalis E, Clin. Orthop. Rel. Res, 193, 246 (1985)

[23] Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemin G, Nat. Biotechnol., 18, 959 (2000)

[24] Kim H, Kim HW, Suh H, Biomaterials, 24, 4671 (2003)

[25] Groeneveld EH, Burger EH, Eur. J. Endocrinol., 142, 9 (2000)

[26] Franz S, Rammelt S, Scharnweber D, Simon JC, Biomaterials, 32, 6692 (2011)

[27] Tapp H, Hanley EN, Patt JC, Gruber HE, Exp. Biol. Med., 234, 1 (2009)

[28] Haidar ZS, Hamdy RC, Tabrizian M, Biotechnol. Lett., 31(12), 1825 (2009)

[29] Katz JM, Nataraj C, Jaw R, Deigl E, Bursac P, J. Biomed. Mater. Res. B Appl. Biomater, 89, 127 (2009)

[30] Takahashi Y, Yamamoto M, Tabata Y, Biomaterials, 26, 4856 (2005)

[31] Sellers RS, Zhang R, Glasson SS, Kim HD, Peluso D, D’Augusta DA, Beckwith K, Morris EA, J. Bone Joint Surg.-Am. Vol., 82, 151 (2000)

[32] Luginbuehl V, Meinel L, Merkle HP, Gander B, Eur. J. Pharm. Biopharm., 58, 197 (2004)

[33] Burdick JA, Mason MN, Hinman AD, Thorne K, Anseth KS, J. Control. Release, 83, 53 (2002)

[34] Bessa PC, Casal M, Reis RL, J. Tissue Eng. Regen. Med., 2, 81 (2008)

[35] Jeon M, Cho J, Kim YK, Jung D, Yoon ES, Shin S, Cho IJ, J. Micromech. Microeng., 24, 025010 (2014)

[36] Jalaja K, James NR, Int. J. Biol. Macromol., 73, 270 (2015)

[37] He JT, Su HB, Li GP, Tao XM, Mo W, Song HY, Int. J. Pharm., 309, 101 (2006)

[38] Mitra M, Mohanty C, Harilal A, Maheswari UK, Sahoo SK, Krishnakumar S, Mol. Vis., 18, 1361 (2012)

[39] Lee JC, Timasheff SN, J. Biol. Chem., 256, 7193 (1981)

[40] Gekko K, Timasheff SN, Biochemistry, 20, 4667 (1981)

[41] Geiger M, Li RH, Friess W, Adv. Drug Deliv. Rev., 55, 1613 (2003)

[42] Choi B, Kim S, Fan J, Kowalski T, Petrigliano F, Evseenko D, Lee M, Biomater. Sci., 3, 742 (2015)

[43] Garcia JT, Farina JB, Munguia O, Llabres M, J. Microencapsul., 16(1), 83 (1999)

[44] Hickey T, Kreutzer D, Burgess DJ, Moussy F, Biomaterials, 23, 1649 (2002)

[45] Mitra A, Wu Y, Recent Pat. Drug. Deliv. Formul., 4, 94 (2010)