Gellan Gum/Chondroitin Sulfate 조성 유착방지제의 개발

김진; 신동일; 이기영; Jin Kim; Dong Il Shin; Ki-Young Lee

Polymer(Korea), Vol.40, No.3, 397-404, May, 2016

DOI10.7317/pk.2016.40.3.397 Export Citation

Gellan Gum/Chondroitin Sulfate 조성 유착방지제의 개발

Prevention of Post-surgical Tissue Adhesions by a Gellan Gum/Chondroitin Sulfate

김진, 신동일, 이기영 ^{1, †}

전남대학교 신화학소재공학과
¹전남대학교 응용화학공학부 & 식품공학 연구소

Jin Kim, Dong Il Shin, and Ki-Young Lee^{1, †}

Department of Advanced Chemicals and Engineering, Chonnam National University, Gwangju 61186, Korea
¹Faculty of Applied Chemical Engineering and Functional Food Research Center, Chonnam National University, Gwangju 61186, Korea

E-mail:

초록

본 연구는 수술 후 유착방지 기능을 갖는 gellan gum(GLG)과 chondroitin sulfate(CH) 조성젤을 만들고 그 효능을 평가하였다. GLG/CH 조성 젤에 phosphate buffered saline(PBS)와 calcium chloride(CaCl2)를 첨가하여 젤을 가교시켰다. 젤의 특성을 확인하기 위해 팽윤성, 젤화도, 젤강도를 측정하고 FTIR로 특성을 확인하였다. Rat에 양성대조군, 음성대조군, 실험군을 넣어 유착방지 효능을 평가하였다. GLG/CH(3:3) 조성 젤은 복막의 조직 유착방지제로 높은 효과를 나타냈고 조직 유착방지막 소재로 활용 가능성을 보였다.

The purpose of this study was to design the gel composed of gellan gum (GLG) and chondroitin sulfate (CH) and to evaluate their efficacy as an anti-adhesion barrier after abdominal surgery. To improve the mechanical properties of the gels, GLG/CH gels were treated in phosphate buffered saline (PBS) and calcium chloride (CaCl2) solutions for cross-linking of polymer chains by divalent cations. The physical and mechanical properties of the gels were confirmed by swelling, gel content, gel strength and fourier transform infrared spectroscopy (FTIR) studies. The positive control, negative control and experiment groups were evaluated for prevention of postoperative adhesion formation in rats. The GLG/CH gel (weight ratio of 3:3) showed high effectiveness for the prevention of peritoneal tissue adhesion. In conclusion, the GLG/CH gel can be a good candidate material as a tissue adhesion barrier.

Keywords:gellan gum;chondroitin sulfate;tissue anti-adhesive;gel

[References]

Boland GM, Weigel RJ, J. Surg. Res., 132, 3 (2006)
Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 57, 40 (1997)
Risberg B, Eur. J. Surg. Suppl., 577, 32 (1997)
Tsujimoto H, Tanzawa A, Matoba M, Hashimoto A, Suzuki S, Morita S, Ikada Y, Hagiwaral A, J. Biomed. Mater. Res. Part B Appl. Biomater., 101B, 99 (2013)
Oh AG, Biomater. Res., 17, 138 (2013)
Kamel RM, Eur. J. Obstet. Gynecol. Reprod. Biol., 150, 111 (2010)
Lee JH, Kim HY, Biomater. Res., 14, 66 (2010)
Lee SA, Kim JS, Kim JS, Hwang JJ, Lee WS, Kim YH, Choi YK, Chee HK, Korean J. Thorac. Cardiovasc. Surg., 43, 596 (2010)
Matricardi P, Cencetti C, Ria R, Alhaique F, Coviello T, Molecules, 14, 3376 (2009)
Oliveira JT, Santos TC, Martins L, Silva MA, Marques AP, Castro Ag, Neves NM, Reis RL, J. Tissue Eng. Regen. Med., 3, 493 (2009)
Ahuja M, Singh S, Kumar A, Inter. J. Biol. Macromol., 53, 114 (2013)
Friedman H, Stonerock C, Lefaivre J, Yost M, J. Invest. Surg., 17, 271 (2004)
Choi EK, Kim HI, Park KR, Nho YC, J. Korean Ind. Eng. Chem., 14(4), 505 (2003)
Verma A, Pandit JK, Trop. J. Pharm. Res., 10, 61 (2011)
Park KR, Kim DP, Nho YC, J. Korean Ind. Eng. Chem., 12(7), 718 (2001)
Hong JH, Choe JW, Kwon GY, Cho DY, Sohn DS, Kim SW, Woo YC, Lee CJ, Kang H, J. Surg. Res., 166, 206 (2011)
Oh AG, Biomater. Res., 17, 138 (2013)
Park JK, Nah JW, Choi CY, Polymer, 39, 480 (2015)
Piattelli A, Scarano A, Russo P, Matarasso S, Biomaterials, 17, 791 (1996)
Sandberg E, Dahlin C, Linde A, J. Oral Maxillofac. Surg., 51, 1106 (1993)

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[1] Boland GM, Weigel RJ, J. Surg. Res., 132, 3 (2006)

[2] Burns JW, Colt MJ, Burgees LS, Skinner KC, Eur. J. Surg. Suppl., 57, 40 (1997)

[3] Risberg B, Eur. J. Surg. Suppl., 577, 32 (1997)

[4] Tsujimoto H, Tanzawa A, Matoba M, Hashimoto A, Suzuki S, Morita S, Ikada Y, Hagiwaral A, J. Biomed. Mater. Res. Part B Appl. Biomater., 101B, 99 (2013)

[5] Oh AG, Biomater. Res., 17, 138 (2013)

[6] Kamel RM, Eur. J. Obstet. Gynecol. Reprod. Biol., 150, 111 (2010)

[7] Lee JH, Kim HY, Biomater. Res., 14, 66 (2010)

[8] Lee SA, Kim JS, Kim JS, Hwang JJ, Lee WS, Kim YH, Choi YK, Chee HK, Korean J. Thorac. Cardiovasc. Surg., 43, 596 (2010)

[9] Matricardi P, Cencetti C, Ria R, Alhaique F, Coviello T, Molecules, 14, 3376 (2009)

[10] Oliveira JT, Santos TC, Martins L, Silva MA, Marques AP, Castro Ag, Neves NM, Reis RL, J. Tissue Eng. Regen. Med., 3, 493 (2009)

[11] Ahuja M, Singh S, Kumar A, Inter. J. Biol. Macromol., 53, 114 (2013)

[12] Friedman H, Stonerock C, Lefaivre J, Yost M, J. Invest. Surg., 17, 271 (2004)

[13] Choi EK, Kim HI, Park KR, Nho YC, J. Korean Ind. Eng. Chem., 14(4), 505 (2003)

[14] Verma A, Pandit JK, Trop. J. Pharm. Res., 10, 61 (2011)

[15] Park KR, Kim DP, Nho YC, J. Korean Ind. Eng. Chem., 12(7), 718 (2001)

[16] Hong JH, Choe JW, Kwon GY, Cho DY, Sohn DS, Kim SW, Woo YC, Lee CJ, Kang H, J. Surg. Res., 166, 206 (2011)

[17] Oh AG, Biomater. Res., 17, 138 (2013)

[18] Park JK, Nah JW, Choi CY, Polymer, 39, 480 (2015)

[19] Piattelli A, Scarano A, Russo P, Matarasso S, Biomaterials, 17, 791 (1996)

[20] Sandberg E, Dahlin C, Linde A, J. Oral Maxillofac. Surg., 51, 1106 (1993)