Amino-functionalized carbon microsphere (NH2-CMS) was successfully synthesized via hydrothermal and amino-functionalized method for electrochemical detection of heavy metal ions for the first time. The as prepared product was characterized by Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). The features of NH2-CMS before and after heavy metal ion adsorption were studied using X-ray photoelectron spectroscopy (XPS). The morphology of synthesized NH2-CMS was investigated by SEM images, and the presence of the amino group was confirmed using FTIR and XPS techniques. The NH2-CMS modified glass carbon electrode (GCE) exhibited an excellent sensitive and selective detection of heavy metal ions simultaneously and individually, including Cd(II), Pb(II), Cu(II) and Hg(II) using square wave anodic stripping voltammetry (SWASV). (C) 2015 Elsevier B.V. All rights reserved.