A glassy carbon electrode modified with cuprous oxide nanopartides-graphene composite film (Cu2O/GR) was prepared and applied to detect acetaminophen (AC). Scanning electron microscopy, energy dispersive X-ray spectroscopy and electrochemical impedance spectroscopy were applied for characterization of the proposed electrode. Cyclic voltammetry and square wave voltammetry were used to investigate the electrocatalysis oxidation of AC on Cu2O/GR. The linearity ranged from 2.0 x 10(-8) to 13 x 10(-8) M with a detection limit of 6.67 x 10(-9) M (S/N = 3) and the calculated sensitivity of 14,397.7 mu A mM(-1) cm(-2). Under optimal conditions, the sensor displays good stability and satisfactory results in real samples analysis. (C) 2015 Elsevier B.V. All rights reserved.