Boron-doped diamond (BDD) is a very promising supporting material used in the construction of biosensors for molecular recognition. The direct immobilization of structurally-organized huge molecules, such as poly-L-lysine (PLL) provides the possibility of determining organic molecules, e.g. nucleic acid bases (e.g. adenine, guanine) or peptides and proteins. This paper describes the direct method for chemical and electrochemical modification of the BDD electrode surface with organic molecules, including the potential application of such modified electrode for detecting selected nucleic acid bases, e.g. adenine and guanine. (C) 2015 Elsevier B.V. All rights reserved.