Mn3O4 nanoparticles decorated with nitrogen-doped reduced graphene oxide (Mn3O4NP/N-rGO) were produced by a facile hydrothermal method. It was further used as a novel sensing material for constructing a sensitive non-enzymatic glucose sensor. Compared with pure Mn3O4, Mn3O4NP/N-rGO showed excellent electro-catalytic activity for glucose oxidation due to the instruction of N-rGO, which exhibited the response current of glucose ca. 20-fold higher than that of the bare glassy carbon electrode (GCE). The proposed sensor showed excellent performances for glucose measurement, including wide linear range of 1.0-329.5 mu M, low detection limit (0.5 mu M, S/N = 3), high sensitivity (0.026 mu A/mu M), fast response time (5 s), and good selectivity to the general co-existing interferences. Such properties would promote the Mn3O4NP/N-rGO as enhanced materials in preparing sensors for chemical and biochemical applications. (C) 2015 Elsevier B.V. All rights reserved.