A non-enzymatic hydrogen peroxide (H2O2) sensor has been developed based on the polydopamine (PDA)/silver nanoparticles (AgNPs) multilayer film (PDA/AgNPs)(n) modified glassy carbon electrode (GCE). The (PDA/AgNPs)(n) multilayer film was fabricated by alternatively dropping dopamine and silver ion solution on GCE taking advantages of the exceptional adhesive and reductive properties of self-polymerized PDA. Electrochemical property of (PDA/AgNPs)(n) modified GCE was characterized by cyclic voltammetry and electrochemical impedance spectroscopy (EIS). Results showed that the as-constructed (PDA/AgNPs) based sensor possesses encouraging catalytic ability for the reduction of H2O2. The sensor has been employed to test real samples and shows strong potential in practical application. (C) 2013 Elsevier B.V. All rights reserved.