Graphene/Nafion nanocomposite film was fabricated on the glassy carbon electrode via a "green" electrochemical reduction route. The X-ray diffraction patterns and UV-Vis spectra confirmed the complete reduction of graphite oxide. The graphene/Nafion nanocomposite film showed high electrochemical performance on the oxidation of 8-hydroxy-2-deoxyguanosine with a wide linear range (0.07-33.04 mu M) and a low detection limit (1.12 nM). Nafion played an important role in suppressing the oxidation current of uric acid, which is a major interferent to the electrochemical detection of 8-hydroxy-2-deoxyguanosine. The experiment results proved that the interference of uric acid to the determination of 8-hydroxy-2-deoxyguanosine can be completely eliminated by uricase. The electrochemically reduced graphene/Nafion nanocomposite film modified electrode will have potential application to the determination of 8-hydroxy-2-deoxyguanosine in real samples. (C) 2013 Elsevier B.V. All rights reserved.