A polyfurfural film modified glassy carbon electrode was prepared by electrochemical polymerization of furfural in acetonitrile solutions and the electrode exhibited a good electrocatalytic activity on the oxidation of polyphenols in 0.1 mol L-1 phosphate buffer solution (pH 6.0). Applications of the polyfurfural film electrode for simultaneous determination of hydroquinone (HQ), catechol (CC) and resorcinol (RS), and for simultaneous determination of resveratrol (RT) and quercetin (QC) were demonstrated. The linear relationship for HQ CC and RS were obtained in the range of 0.5-10, 1-40 and 0.5-10 mu mol L-1, respectively. The detection limits (S/N = 3) were 0.04 mu mol L-1, 0.08 mu mol L-1 and 0.06 mu mol L-1, respectively. While for RT and QC, the oxidation peak current of RT was linear with the concentration of RT from 0.8 to 8 mu mol L-1 and the detection limit (S/N = 3) was 0.4 mu mol L-1. Meanwhile the oxidation peak current of QC was linear with the concentration of QC from 0.8 to 12 mu mol L-1 and the detection limit (S/N = 3) was 0.3 mu mol L-1. Simultaneous determination of relevant polyphenols without interference with each other was realized by the polyfurfural film modified electrode. The electrodes are expected to be used as sensors for the three dihydroxybenzene isomers. The electrode also has the potential application of rapid determination of RT and QC in wine and food. (C) 2012 Elsevier B.V. All rights reserved.