Poly (3,4-ethylene dioxythiophene)/sulphated beta-cyclodextrin (PEDOT/S-beta-CD) films, deposited onto gold working electrodes, were investigated for ascorbic acid (AA) and catecholamine detection using cyclic voltammetry, hydrodynamic voltammetry and amperometry. The thin PEDOT/S-beta-CD films were fabricated via three electropolymerisation cyclic voltammetry cycles, on gold working macroelectrodes. A limit of detection (LoD) of 1.3 x 10(-7) M was determined at PEDOT/S-beta-CD modified gold electrodes, via cyclic voltammetry, for dopamine (DA) in the presence of 1 x 10(-3) M of AA in human urine samples. (C) 2011 Elsevier B.V. All rights reserved.