A novel electrochemical system for the sensitive determination of cis-jasmone on an electrodeposited Nafion film modified glassy carbon electrode (e-Nafion/GCE) in the presence of cetyltrimethylammonium bromide (CTAB) was described. The redox mechanism of cis-jasmone on e-Nafion/GCE was studied by voltammetry, chronocoulometry and infrared spectroscopy. The property and interface behavior of the e-Nafion film were characterized by electrochemical impedance spectrometry. The standard rate constant k(0), the diffusion coefficient D and the surface coverage Gamma were calculated and discussed. Under optimal working conditions, the anodic stripping peak current of cis-jasmone linearly increased with its concentration in the range of 6.0 x 10(-7) to 1.0 x 10(-4) mol/L, with a detection limit of 4.0 x 10(-7) mol/L. The proposed method was applied to the determination of cis-jasmone in spikelet samples of rice and the good recoveries showed that it may have potentially valuable applications. (C) 2011 Elsevier B.V. All rights reserved.