Alginate/pectin hydrogel microspheres were prepared by extrusion based on a vibrating technology to encapsulate bacteriocin-producing lactic acid bacteria. Effects of both alginate/Pectin (A/P) biopolymers ratio and physiological state of Lactococcus lactis subsp. lactis (exponential phase, stationary phase) were examined for nisin release properties, L. lactis survival and beads physico-chemical properties. Results showed that A/P composites were more efficient to increase beads properties than those formulated with pure alginate or pectin. Association of alginate and pectin induces synergistic effect which improves microbeads mechanical properties. FTIR spectroscopy confirms possible interactions between alginate and pectin during inter-penetrating network formation. Physiological state of bacteria during encapsulation process and microbeads composition (A/P ratio, enrichment of internal medium with nutrients) were determining factors for both bacteria viability and bacteriocin release. Of the several matrices tested A/P (75/25) with glucose-enriched M17 gave the best results when L lactis was encapsulated in exponential state. (C) 2016 Elsevier Ltd. All rights reserved.