The present study examined bioleaching of heavy metals from mobile phone printed circuit boards (MPPCBs) using adapted Acidithiobacillus ferrooxidans. The adaptation phase (10% v/v inoculum) began with 1 g PCB powder per 1L culture medium and continued for 55 days to a level of 20 g PCB powder per 1L medium. The factors that examined for metal bioleaching were initial pH, initial Fe3+ concentration, pulp density and particle size. To maximize simultaneous extraction of Cu and Ni from MPPCBs, these factors were optimized under a multi-objective optimization strategy using the central composite design of response surface methodology. Two modified quadratic models were selected to predict the interactions and behavior of the influence parameters on the bioleaching of Cu and Ni from e-waste samples. An initial pH of 1, initial Fe3+ concentration of 4.18 g/l, pulp density of 8.5 g/l and particle size of 114.02 mu m (#100 mesh) were determined as the optimal conditions. Under these conditions, 100% extraction of Cu and Ni was achieved. The pH, Eh, bacterial count, and Fe3+ concentration were varied over time and measured under optimal conditions. (C) 2015 Elsevier B.V. All rights reserved.