Separation and Purification Technology, Vol.162, 68-76, 2016
Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit
The aim of the present study is to investigate the effects of solvent type (ethanol, methanol, acetone and water), acetone concentration (20-100%, v/v), solvent acidity (0-2 N), time (30-450 min) and temperature (25-70 degrees C) on the extraction of total phenolic compounds (TPC), total flavonoid compounds (TFC) and on the antioxidant capacity: 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity (DPPH-RSA) and ferric reducing power (FRP) of peach fruit (Prunus persica L.) using single factor experiments approach. All the studied extracting conditions showed significant effect (p < 0.05) on TPC, TFC, DPPH-RSA and FRP. On the basis of TPC and antioxidant activity parameters, the best extraction conditions were 60% acetone without acidification for 180 min at 25 degrees C. Based on these optimized conditions, high content of TPC, DPPH-RSA and FRP of peach extracts were obtained with values of 363 GAE/100 g, 48% percentage of inhibition and 317 AAE/100 g, respectively. Good, positive or negative, Pearson correlation coefficients were found between TPC, TFC DPPH-RSA and FRP of peach extracts, especially under the influence of solvent type, solvent concentration and acidity extraction conditions. (c) 2016 Elsevier B.V. All rights reserved.
Keywords:Peach (Prunus persica L.);Phenolic compounds extraction;Antioxidant activity;Solvent;Time;Temperature