This work aimed to develop voriconazole in situ gelling ocular inserts loaded with niosomal suspension. Niosomes and mixed niosomes were prepared using span 40 and span 60 with pluronic L64 and pluronic F127. The entrapment efficiency percentages (EE%), mean vesicle size, polydispersity index (PI), zeta potential and invitro drug release of these niosomes were evaluated. F3-mixed niosomes prepared with span 60 and pluronic L64 was selected, due to its highest EE; optimum vesicle size with smallest PdI and suitable release pattern of the drug (63% after 8h). In situ ocular inserts were prepared using sodium carboxymethylcellulose (CMC Na) and sodium alginate (ALG) and characterised for surface morphology, surface pH, water uptake, mucoadhesion and invitro release. ALG in situ ocular insert (S-2) was selected for further invivo evaluation of the ocular irritation and drug pharmacokinetics in the aqueous humour of rabbit's eyes. S(2)in situ gelling ocular insert was non-irritant and showed significantly (p<0.01) higher C-max, delayed T-max and increased bioavailability.