We describe molecularly imprinted polymer (MIP) microprobes for manipulating neurological function by the thermoregulatory process. Some target and disease areas show raised temperature than normal temperature which are involved in neuronal diseases, such as Alzheimer's, Parkinson's and Hunting ton's diseases. As well thermodynamic parameters can be obtained in the environments in vitro, which that the assay involves a temperature variable, at or beyond body temperature. We applied for studying molecular aspects of hypothalamus regulated physiology and behaviors. Steady-state binding was detected by fluorescence and Boltzmann energies-based fluorescence emission showed interactions of clozapine with altered energy levels. Dynamic spectra revealed dramatic effects on the fluorescence of the drug in dopamine imprint of MIPs exposed rat hypothalamic membranes at different temperatures. The MIPs that cooperatively occupied and provided the response to the neural proteins were not likely to be involved in the binding of clozapine within the cavity. The results indicated that the dynamics on the surface of proteins exposed to the drug-MIP with assembly, mediated specific function through temperature-dependent molecular interaction in the hypothalamus. Thus, this approach is applicable to processes of key importance of controlling the energy homeostasis and, plausibly, to selective signaling can affect in plasticity of the neural response. (C) 2015 Elsevier Ltd. All rights reserved.