Design of the transcriptionally-fused protein MoHhaI-EGFP, composed of bacterial DNA-methyltransferase MoHhaI and enhanced green fluorescent protein (GFP) is described. The mentioned MoHhaI-EGFP was expressed in Escherichia coli ER1821 and purified by affinity chromatography on Ni-NTA agarose. According to expectations MoHhaI-EGFP fused protein retained significant features of corresponding original proteins: the ability to transfer methyl group to the C5 carbon atom of internal cytosine in CGCG site and absorption-emission spectral characteristics. The created transcriptionally-fused protein MoHhaI-EGFP could be used in various experiments in molecular biology. (C) 2014 Elsevier Ltd. All rights reserved.