The purpose of the present study is to find the conditions allowing to reach the highest 24 h-yield (24 h-eta) for the synthesis of mannosyl myristate catalyzed by the immobilized lipase B from Candida antarctica (Novozym (R) 435) in the ionic liquid (IL) [Bmpyrr][TFO] (1-butyl-1-methylpyrrolidinium trifluoromethanesulfonate). A full factorial design (FFD) was used in order to study the influence of three variables (temperature, mannose/vinyl myristate ratio and total substrate quantity) on the 24 h-eta. This design led to a model based on a second order polynomial response function. The resulting predicted contour plots have shown that the highest 24 h-eta should be obtained with high temperatures, low sugar/vinyl ester molar ratio and intermediate total substrate quantities (mmol). The model has been successfully verified and experimentally confirmed at the optimal conditions of 80 degrees C, substrate molar ratio of 1/10 and total substrate quantity of 0.26 mmol leading to the highest predicted 24 h-eta of 72.2%. (C) 2013 Elsevier Ltd. All rights reserved.