A novel process strategy based on particulate bioprocessing has been developed for the production of value-added chemicals and biofuels. The process, which involves two main steps, fungal fermentation and discontinuous extraction, leads to the production of generic fermentation feedstocks from cereals. Partially pearled whole wheat grains were used as substrate for the growth of Aspergillus awamori in a packed bed bioreactor. Water was trickled through the bed of particles intermittently every 6 h to extract glucose and other nutrients and to maintain moisture and temperature levels. The feedstocks obtained through this system have been used for subsequent fermentations by Wautersia eutropha to produce the biodegradable plastic PHB (polyhydroxybutyrate) and by Saccharomyces cerevisiae for ethanol production. These preliminary results demonstrate the potential suitability of the novel concept of particulate bioprocessing in the development of biorefineries. (C) 2009 Elsevier Ltd. All rights reserved.