Lipase from Yarrowia lipolytica is an enzyme that presents numerous potentialities for biotechnological applications. This work describes the development of powders obtained by atomization of supernatants lipase from Y lipolytica LGx6481. Two formulations were studied: one formulation with skim milk powder and gum arabic, and the other with maltodextrin, calcium chloride and gum arabic. After drying, powders were stored at 4 and 20 degrees C in aluminium hermetic bags to evaluate their stability over a period of one year. The influence of water activity and glass transition temperature (T.) on the powder's storage were also studied. (c) 2007 Elsevier Ltd. All rights reserved.