The present study deals with the development of purification and separation processes required to produce the highly purified succinic acid from the fermentation broth produced by recombinant microorganism, Mannheimia succiniciproducens. The newly developed process consists of the pretreatment process such as reactive extraction and vacuum distillation step and the crystallization process for the highly purified succinic acid production. By-produced acids were effectively removed by the reactive extraction as a primary separation. In addition, the crystallization was applied without adding any salts to produce highly purified succinic acid. The purified succinic acid, with 99.8% purity and 73.1% yield rate was obtained through this newly developed purification process. (c) 2006 Elsevier Ltd. All rights reserved.