Two specimens of Euphrasia pectinata collected from different regions of northern Anatolia were studied to determine their iridoid and phenylethanoid glycosides. Four iridoid glucosides, aucubin (1), euphroside (2), ixorodoside (3), and boschnaloside (4), and two phenylethanoid glycosides, acteoside (5) and leucosceptoside A (6), were isolated, and characterized from the aerial parts of E. pectinata collected from Zigana pass (Trabzon). From the aerial parts of E. pectinata collected from Kartalkaya (Bolu), a phenethyl alcohol glycoside, decaffcoylacteoside (7), was isolated in addition to previously isolated compounds. The structures of the isolated compounds were established by spectroscopic evidence.