The degree of encapsulation of 5-fluorouracil (5-FU) and vinorelbine (VIN) to dipalmitoylphosphatidylcholine (DPPC) liposomes was estimated using UV-vis spectroscopy. The phase transition temperature was determined by the differential scanning calorimetry (DSC). 5-FU incorporates in more extend to liposomes than VIN and affects phase transition temperature of phospholipide forming liposomal membrane. There is only a slight influence of VIN on the 5-FU encapsulation in liposome vesicle while 5-FU affects significantly the VIN content in the liposome vesicles. The binding sites of VIN and 5-FU in HSA determined by fluorescence quenching method were confirmed by computer simulation.