We prepared multi-bilayered films consisting of methacrylate polymer having azobenzene side chain groups (PMAz6Mc), polyvinyl alcohol and lasing dye. We investigated effects of molecular orientation of azobenzene moieties on the lasing behavior by measuring laser emission of the multi-bilayered films with/without polarized visible light irradiation to cause trans-cis-trans photoisomerization of azobenzene moieties. After irradiation of the polarized visible light, significant dependence of polarization direction of the laser emission intensity was observed, corresponding to the change in the molecular orientation of azobenzene moieties.