We report a new method for the detection and measurement of the chirality of liquid crystal compounds. The method is based on the determination of an azimuth of a disclination line in a -cell, with the director aligning unidirectionally at one side and circularly at another one. Experimental observation of disclination rotation was performed with a composition of ZLI-4801-0000 liquid crystal and dopants of opposite-sign chirality (R-2011, S-2011, 7-dehydrocholesterol). Dynamic behavior of the disclinaton line was revealed under the influence of continuous illumination by actinic UV radiation.