We demonstrated the field-directed diffusion of charged phospholipids in supported bilayer membranes into distributed partitions in a diffusion cell. The balance between the field-induced drift and the thermal Brownian motion generates the concentration gradient of the charged lipids from partition to partition under an external electric field applied longitudinal to the partition walls. The concentration gradient across the partitions was found to primarily depend on the pore width and the distance between the pore and the partition wall. Our field-directed diffusion approach provides a powerful tool for constructing various spatially addressed membrane arrays.