The interactions between femtosecond laser and collagen films have been investigated. Collagen was obtained in our laboratory from tail tendons of young albino rats. Thin collagen films were obtained by casting of collagen solution onto glass plates. The changes on film surfaces after femtosecond laser irradiation were examined using scanning electron microscopy. The laser irradiation caused an expansion of materials above the surface of collagen films. The obtained results are considered in terms of possible ablation mechanisms and compared with recent results obtained with nanosecond laser pulses.