The self-assembling behavior of a conjugated triblock copolymer (PEGMA-b-PF-b-PEGMA) containing an oligofluorene (PF) block and poly[penta(ethylene glycol) methyl ether methacrylate] (PEGMA) blocks was investigated. Due to the amphiphilic nature of PEGMA-b-PF-b-PEGMA, the micellar aggregates of PEGMA-b-PF-b-PEGMA were induced in selective solvents. Photoluminescence spectra of PEGMA-b-PF-b-PEGMA were also measured and compared with the data measured from the SAXS and TEM measurements.