Subcritical water is a natural and green way for extraction of plant biomass components. Here, subcritical water was employed as a media to extract -glucan from barley (Hordeum vulgare L.) at 130-170 degrees C and 10 MPa. MALDI TOF-MS revealed that main massed peaks of water soluble products were distributed at 500-2900 m/z with a peak to peak mass difference of 162 m/z, consistent with the repeating unit of -glucan. At 170 degrees C, all amount of water soluble -glucan has been extracted. Next, the liquid fraction products were atomized directly to produce microsphere particles.