In this paper, limiting factors for increakd use of biomethane as a transportation fuel are studied. The aim of this research is to recognize and estimate the limiting factors for biomethane utilization in the transportation sector. The limiting factors are studied by using calculation models from Life cycle perspective and literature reviews. According to the results, the main limiting factors can be classified into the following categories: production potential, technology, economy or policy. For biomethane utilization in Finland, the main limiting factors seem to be the lack of distribution infrastructure in northern parts of the country and the uncertain economical feasibility for agricultural biomass producers and for vehicle owners. From the political perspective, the external costs for petrol operated vehicles are higher than for biomethane operated vehicles. Reductions from the external costs could be used by political decisions as a base to support the growth of biomethane in the transportation sector which could lead to GHG emission reductions. A similar systematic approach can also be used to study limiting factors for other transportation energy systems. (C) 2015 Elsevier Ltd. All rights reserved.