An experimental investigation for sensitive spectrophotometric detection of explosive, 2,6-bis(picrylamino)pyridine (BPAP) using dispersive liquid-liquid microextraction was carried out. Based on this procedure, which is a dispersive-solvent-free technique, the extractant is dispersed in the aqueous sample solution using Aliquat 336 (acted as disperser agent and carrier to extraction solvent) and monitored with microvolume UV/Vis spectrophotometer. The effect of different variables such as pH, concentration of sodium hydroxide, type and volume of extraction solvent, concentration of Aliquat 336 solution and coexisting substances were systematically investigated and optimized. Interference tests showed that the developed method has a good selectivity and could be used conveniently for determination of explosive analyte. The proposed method is capable of determining BPAP over a range of 2.0-150.0ngmL(-1) with a limit of detection 1.0ngmL(-1). Relative standard deviations (RSD) for 20.0 and 80.0ngmL(-1) of BPAP were 3.3 and 1.2% (n=10), respectively. This colorimetric method was applied to determine BPAP in different water and soil samples.