The effect of the temperature on strain induced crystallization (SIC) of natural rubber (NR) is studied by in situ wide angle X-rays scattering (WAXS) experiments performed along different thermo-mechanical paths (cyclic deformation at different temperatures or crystallization and melting in the deformed state). The crystallinity index (Cl), the average crystallite size (L-200) and the average volume of the crystallites (V) increase (decrease) similarly if the sample is cooled (heated) in the deformed state or stretched (unstretched) at fixed temperature. These experiments are analyzed through a thermodynamic description assuming that SIC is controlled by successive nucleation of crystallite populations. Such description predicts that for a stretching ratio lambda above (below) 4 and a temperature above (below) room temperature, crystallization first occurs in chains with the highest (lowest) network chain density, leading to the smallest (largest) crystallites. (C) 2015 Elsevier Ltd. All rights reserved.