In this study, we examined about effectives of the drug release by used glutaraldehyde amount and molecular weight varieties of chitosan in DDS. The release experiments of chitosan microcapsules containing norfloxacin were operated in the phosphate buffer solutions of pH 7.4 and 37℃, and its results were as follows. In the linearly release time of drug, chitosan microcapsules (Mw 350,000) of used glutaraldehyde 1 mL and 2 mL were 6.0 and 8.1 days, and chitosan microcapsules (Mw 190,000) of used glutaraldehyde 1 mL and 2 mL were 4.5 and 6.4 days relatively. These results showed more effective drug releases in the increments of molecular weight and glutaraldehyde. In short, the formulation allows chitosan microcapsules to suppress the burst effect of the drug release mechanism, which led to the controlled release pattern and microcapsules.