Chiral separation of seven commonly used 1,4-dihydropyridines (1,4-DHP) was achieved by supercritical fluid chromatography (SFC) on a immobilised polysaccharide chiral selectors coated with cellulosetris(3,5-dichlorophenylcarbamate) (Chiralpak IC). In this method, isopropanol was used as a modifier and a maximum resolution of 13.38 was resulted. Nimodipine content of actual samples was determined through two-phase hollow fibre-based liquid-phase microextraction (2p-HF-LPME). Under optimal conditions, the limits of detection of the two nimodipine enantiomers were 0.3 and 0.5 mu g cm(-3). Recoveries of 80.0-99.8% were achieved. The developed SFC technique coupled with 2p-HF-LPME is a rapid, effective and environment-friendly method for separating and quantifying 1,4-DHP enantiomers. (C) 2015 Elsevier B.V. All rights reserved.