The thermodynamic stability and kinetic barriers separating protein conformations under native conditions are critical for proper protein function and for understanding dysfunction diseaSes of protein conformation. Traditional methods to probe protein unfolding and folding employ denaturants and highly non-native conditions, which may destabilize intermediate species or cause irreversible aggregation, especially at the high protein concentrations typically required. Hydrogen exchange (HX) is ideal for detecting conformational behavior under native conditions without the need for denaturants, but detection by NMR is limited to small highly soluble proteins. Mass spectrometry (MS) can, in principle, greatly extend the applicability of native-state FIX to larger proteins and lower concentrations. However, quantitative analysis of HXMS profiles' is currently limited by,ekperimental and theoretical challenges. Here we address both limitations, by proposing an approach based on using standards to eliminate the systematic experimental artifacts in HXMS profiles; and developing the theoretical framework to describe HX behavior acress all regimes based on the Linderstrom-Lang formalism We demonstrate proof of principle bya practical application to native-state HX of a globular protein. The framework and the practical tools developed advance the ability of HXMS to extract therinodynamic and kinetic Conformational parameters of proteins under native conditions.