In this work neutral tyrosine has been generated in the gas phase by laser ablation of solid samples, and its most abundant conformers characterized through their rotational spectra. Their identification has been made by comparison between the experimental and ab initio values of the rotational and quadrupole coupling constants. Both conformers are stabilized by an O-H center dot center dot center dot N hydrogen bond established within the amino acid skeleton chain and an additional weak N-H center dot center dot center dot pi hydrogen bond. The observed conformers differ in the orientation of the phenolic -OH group.