An edible antimicrobial film was prepared with defatted soybean meal (DSM) and the lactoperoxidase system (LPOS) (LPOS-DSM films) by heat pressing a film-forming mixture at 90 degrees C and 40 MPa for 3 mm. The film was applied to ham slices and the concentration of hypothiocyanite (OSCN-) was monitored over time. Based on the data obtained, a model was developed that predicted that a film containing OSCN- at 0.66 mg/g could provide 42.9 h of protection against 2.8 log CFU/g. of Salmonella Typhimurium. A Monte Carlo simulation-based analysis estimated that coating ham with the LPOS-DSM film would decrease the probability of occurrence of >1 case of salmonellosis per year by 90.7%, associated with consumption of ham. The methods of quantitative risk assessments developed in this study may also be useful for evaluating other edible antimicrobial films. (C) 2015 Elsevier Ltd. All rights reserved.