In this work, we aimed to select suitable non-repressing carbon sources for the expression of promoters derived from the alcohol oxidase (AOD) isozyme genes, P-MOD1 and P-MOD2, during the growth of Pichia methanolica. Our results revealed that xylose is the best non-repressing carbon source for heterologous gene expression using both P-MOD1 and P-MOD2, and that glycerol is also a suitable carbon source with by which the on/off of P-MOD2 expression can be controlled. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.