The design of observers and output feedback stabilising controllers for continuous-time strict-feedback systems with sampled observation is considered. First two types of observers are designed. One is a discrete-time semiglobal and practical reduced-order observer for the exact model and the other is a continuous-time semiglobal and practical full-order observer for continuous-time strict feedback systems with sampled observation. Then by combining the designed continuous-time observers and continuous-time state feedback laws that are continuous, zero at the origin, and uniformly globally asymptotically stabilise continuous-time systems, output feedback semiglobally practically uniformly asymptotically stabilising controllers are constructed. Numerical examples are given to illustrate the proposed design of observers and output feedback controllers.