Fatty acid alkanolamides (FAAA) at lipid derivatives with industrial applications as biosurfattants and bioltbricants. Although conventionally produced from vegetable oils, use of alternative renewable sources-that do not compete with the food supply chain, such as microalgae, is desirable. We studied the production of FAAA through direct in situ afnidation of algal biomass or by amidation of fatty,acid methyl esters (FAME) recovered from in situ transesterification of alpae. In situ tfansesterification resulted in spontaneous, formation of a distinct FAME phase, which could be easily recoveied and converted to FAAA. With this two-step transesterification-followedby-amidation method, >95% of algal lipids were recovered as FAAA products. In situ amidation Jolla not result in a separate product phase, likely because of the amphiphilit nature of the product. However, extraction with ethyl acetate allowed recovery of nearly 90% of the biomass lipids as FAAA after in situ athidation.