Linear regression equations for dry cell weight versus optical density (OD680) for 16 strains of microalgae were developed. Carbon contents of the green algae and diatoms were between 40.56-49.62% and 25.74-41.7%, respectively. The ventilation (especially the addition of CO2) and high light intensity significantly improved the microalgae growth rates and biomass amounts. Dry cell weight of eight strains up to 2.119-3.452 g.L-1 were achieved when ambient air containing 5% CO2 (v/v) and high light intensity were applied. The microalgal lipids contained fatty acid methyl esters mainly with C16:0, C18:1, C18:2, and C18:3, which were considered suitable for producing biodiesel.