A major barrier that limits degradation of the lignocellulosic materials during biogas production is the structure itself. Without pretreatment, enzymatic attack during hydrolysis is not effective and leads to a poor yield of biogas. Thus, in this study, the effect of extrusion as pretreatment on wheat straw and deep litter was evaluated. Five screw configurations of the extruder were tested, namely, mild kneading, long kneading) reverse, kneading and reverse, and kneading with reverse. Sugar availability and biogas potentials from extruded samples were examined. Energy consumption, barrel temperature, and residence time of samples during extrusion were measured. The results showed an increment in methane yields of about 4-29 and 1-16% of extruded samples after 28 and 90 days of anaerobic digestion, respectively. A strong positive Cori-elation (R-2 = 0.70) was observed between ultimate sugar availability and methane yields at 28 days. Increases in sugar availability (7-42%) accelerate degradation of the biomasses at the early digestion phases, resulting in higher yield of methane. Extrusion was less effective on deep litter because of the soft texture and possibility of sugar hydrolysis during storage.