-Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the -agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the -agonists. The chip was fabricated from two glass plates (64 mmx32 mm) with microchannels of 200 m width and 100 m depth. The detection limits were achieved at 2.0x10(-8)mol/L of ractopamine, 1.0x10(-8)mol/L of terbutaline and 5.0x10(-7)mol/L of salbutamol. In this report, our method was applied for determination of the -agonists in swine hair from three different sources with satisfactory results.