A ceramic-based miniature biosensor was designed and constructed through the immobilization of laccase in an electrochemically synthesized copolymer N-hexylacridone derivative, based on low temperature co-fired ceramics technology (LTCC). This sensing system can be suitable for the permanent monitoring of water solutions (i.e. presence of phenolics). The laccase, from Cerrena unicolor, was successfully immobilized (by adsorption) on the surface of the thin polymer film built from 2,7-bis (thiophene)-N-hexylacridone, covering the microreaction chamber. The detection process was based on the oxidation of substrate in the presence of the enzyme-laccase. The response of the biosensing system was evaluated in the presence of ABTS (ammonium salt of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)), used as an enzymatic assay substrate. The system exhibited clear catalytic activity, and the substrate could be optically determined. Considering the fact that the immobilization strategy showed high efficiency, the obtained results suggest that this method for phenoloxidase immobilization has great potential to enable the high throughput fabrication of bioelectronic devices. (C) 2015 Elsevier Ltd. All rights reserved.