Graphene oxide decorated with silver nanoparticles (GO-Ag) was prepared by anodic dissolution of silver in the aqueous dispersion of GO. The composites were characterized by XRD, XPS, TEM, AFM, and Raman spectroscopy. The electrooxidation of glucose on GO-Ag modified electrodes have been tested by cyclic voltammetry and chronoamperometry. The detail mechanism of redox processes on the GO-Ag electrodes has been studied. A few mu g loading of silver has demonstrated to give current in mu A for the mM concentration of glucose. A linear relationship between peak height in the voltammograms and glucose concentration in the range 1-14 mM has been proposed for amperometric detection of glucose. From the results, the detection limit for glucose sensing is estimated to be as small as 4 mu M. The selectivity for glucose in presence of interfering molecules viz. ascorbic and uric acids is tested. Proof-of concept is presented by carrying out the measurements in real human blood samples. (C) 2015 Elsevier Ltd. All rights reserved.