Antifouling coatings are used extensively on vessels and underwater structures. Conventional antifouling coatings contain toxic biocides and heavy metals, which may induce unwanted adverse effects such as toxicity to non target organisms, imposex in gastropods and increased multiresistance among bacteria. Therefore, enzyme-based coatings could be a new alternative solution. A H2O2-producing bienzyme system was developed in this study. H2O2 can be produced from starch by the cooperation of a-amylase and glucose oxidase, which promotes the hydrolysis of polymeric chain and oxidizes the glucose to produce H2O2, respectively. The encapsulated bienzyme (A-G@BS) exhibits enhanced stabilities of thermal, pH, recycling and tolerance of xylene. The A-G@BS-containing coating releases H2O2 at rates exceeding a target of 36 nmol.cm(-2).d(-1) for 90 days in a laboratory assay. The results demonstrate that the method is a promising coating technology for entrapping active enzymes, presenting an interesting avenue for enzyme-based antifouling solutions. (C) 2015 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. All rights reserved.