Solute transport behavior across liquid-liquid interfaces plays important roles in many chemical engineering processes and usually occurs in he presence of surfactants. In this work, we report a full all-atom molecular dynamics simulation study of dimethyl sulfoxide (DMSO) crossing a water/hexane interface in different concentrations of sodium dodecylsulfate (SDS). By analyzing various properties, ranging from the configuration and energetic behaviors to the dynamic characteristics, we conclude that the presence of SDS has multiple non-trivial effects: (1) the association of SDS at the interface drives the interfacial region wider and decreases the interfacial tension with an almost linear dependence on SDS concentration; (2) higher SDS concentrations result in both a lower self-diffusion coefficient for DMSO and a smaller resistance force to DMSO moving out of the interfacial region, causing the exchange of DMSO between the interface and bulk region to become more frequent; and (3) the transition of the orientation preference of DMSO molecules passing from the interface to the water-rich phase vanishes. Current simulation studies examining the molecular level provide helpful insights for understanding the influence of surfactants on solute transport. (C) 2015 Elsevier Ltd. All rights reserved.