Biohydrogen production in fixed-bed reactors often leads to unstable and decreasing patterns because the excessive accumulation of biomass in the bed negatively affects the specific organic loading rate (SOLR) applied to the reactor. In this context, an innovative reactor configuration, i.e., the continuous multiple tube reactor (CMTR), was assessed in an attempt to better control the SOLR for biohydrogen production. The CMTR provides a continuous discharge of biomass, preventing the accumulation of solids in the long-term. Sucrose was used as the carbon source and mesophilic temperature conditions (25 degrees C) were applied in three continuous assays. The reactor showed better performance when support material was placed in the outlet chamber to enhance biomass retention within the reactor. Although the SOLR could not be effectively controlled, reaching values usually higher than 10 g sucrose g (1) VSS d (1), the volumetric hydrogen production and molar hydrogen production rates peaked, respectively, at 1470 mL H-2 L (1) d (1) and 45 mmol H-2 d (1), indicating that the CMTR was a suitable configuration for biohydrogen production. (C) 2015 Elsevier Ltd. All rights reserved.