Two North American tall perennials, Sida hermaphrodita L Rusby and Silphium perfoliatum L have been recognized in Europe as high-yielding novel bioenergy species. While the latter is recommended for biogas production in Germany, the ligno-cellulosic stems of S. hermaphrodita are widely used as a solid fuel in Poland. Since information on the adaptation of the species to drought and heat and interactions with the CO2 fertilization effect were lacking, growth chamber experiments were performed with seedgrown and established plants. A full factorial combination of two temperatures, two water levels and two CO2 levels was applied using the long-term seasonal climate of southwestern Germany as a reference. Non-destructive parameters (length, phenology and senescence) and five harvests served to identify treatment effects on growth and allocation patterns. Shoot material was subjected to chemical and bioenergetic analyses (methane production and energy contents) and NIR-spectroscopy. While seedlings showed stronger growth responses to the treatments than established plants, interspecific differences of the responses were mostly related to allocation patterns and senescence. S. perfoliatum, which has a greater proportion of leaf mass was able to profit from CO2 fertilization even under dry conditions, while in S. hermaphrodita such effects were absent. Chemical quality (crude protein, ash, fat and fibre) was mainly affected by the reduced water supply and energetic values in S. hermaphrodita and specific methane yields of S. perfoliatum tended to be lower. NIR spectra showed a good representation of percentage leaf mass, which in both species determines the quality of the shoot. (C) 2015 Elsevier Ltd. All rights reserved.