In this paper, a systematic investigation of the influence of pressure on electrolytic conduction of conical nanopores in track-etched polyimide (Kapton) films is described. The effect of pressure is shown to be dependent on the pore orifice size and the rectification ratio of nanopore. For larger Kapton nanopores, the disruption of cation and anion equilibrium distributions within the nanopore caused by pressure resulted in the decrease of the rectification, but the pressure effect on the I-V also exhibited some differences for larger nanopores with different diameter. For smaller nanopores, pressure had a negligible influence on rectification. (C) 2015 Elsevier B.V. All rights reserved.